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General Information

Document type
  • Peer-reviewed journal article
Study Type
  • composition
  • genetic analysis
GE organism
  • maize
GE trait
  • quality
Country
  • China

Results

Efficacy
  • positive effect

Seed-specific expression of the lysine-rich protein gene sb401 significantly increases both lysine and total protein content in maize seeds Open Access

Yu, J; Peng, P; Zhang, X; Zhao, Q; Zhu, D; Sun, X; Liu, J; Ao, G
Food and Nutrition Bulletin. 2005 December. 26(4):427-31

Link to full text (open access, freely available)

PMID: 16465991 ISSN: 0379-5721

Abstract

The sb401 gene from potato (Solanum berthaultii) encoding a pollen-specific protein with high lysine content was successfully integrated into the genome of maize plants, and its expression was correlated with increased levels of lysine and total protein content in maize seeds. A plasmid vector containing the sb401 gene under the control of a maize seed-specific expression storage protein promoter (P19z) was constructed and introduced into maize calli by microprojectile bombardment. The integration of the sb401 gene into the maize genome was confirmed by Southern blot analysis, and its expression was confirmed by Western blot analysis. Quantification of the lysine and protein contents in R1 maize seeds showed that, compared with the nontransgenic maize control, the lysine content increased by 16.1% to 54.8% and the total protein content increased by 11.6% to 39.0%. There were no visible morphological changes in the vegetative parts and seeds of the transgenic maize plants. Lysine and protein analysis of the transgenic maize grains showed that the levels of lysine and total protein remained high for six continuous generations, indicating that the elevated lysine and total protein levels were heritable. These results indicate that the sb401 gene could be successfully employed in breeding programs aimed at improving the nutritional value of maize.

Keywords

lysine content; Lysine-rich proteinSB401 gene; Microprojectile bombardment; Protein content; Transgenic plants; Zea Mays L

Funding

Funding source
  • 863 High Tech Program
  • National Specific Project of Trangenic Plant Research and Commercialization
Funding country
  • China
Funding type
  • government

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Cite this study

MLA

Yu, J, P Peng, X Zhang, Q Zhao, D Zhu, X Sun, J Liu, G Ao. "Seed-specific expression of the lysine-rich protein gene sb401 significantly increases both lysine and total protein content in maize seeds." Food and Nutrition Bulletin 26.4 (2005): 427-31. Web. 24 Sep. 2018.

APA

Yu, J., Peng, P., Zhang, X., Zhao, Q., Zhu, D., Sun, X., Liu, J., & Ao, G. (2005). Seed-specific expression of the lysine-rich protein gene sb401 significantly increases both lysine and total protein content in maize seeds. Food and Nutrition Bulletin, 26(4), 427-31.

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