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General Information

Document type
  • Peer-reviewed journal article
GE organism
  • soybean
GE trait
  • herbicide tolerance
Country
  • Belgium

Characterisation of the Roundup Ready soybean insert

Windels, P; Taverniers, I; Depicker, A; Van Bockstaele, E; De Loose, M
European Food Research and Technology. 2001 August. 213(2):107-112

Link to full text (journal may charge for access)

DOI: 10.1007/s002170100336 ISSN: 1438-2377

Abstract

In this article we describe the isolation and characterisation of the junction between insert DNA and plant DNA in the transgenic Roundup Ready soybean line event 40-3-2. Our results establish that during integration of the insert DNA several rearrangements occurred at the 3′ NOS junction and that the genomic plant DNA at the pre-integration site may have been rearranged. These findings highlight the utility of characterising junction regions to fulfil the request for information regarding which DNA sequences have been incorporated in commercialised transgenic lines. Furthermore, the characterisation of junction regions is, in our opinion, the method of choice to support method development for detection and identification of plant biotechnology-derived products.

Keywords

Roundup Ready soybean, Junction fragment, Rearrangements, GMO, Identification

Funding

Funding source
  • European Commission
  • Federal Office for Scientific, Technological and Cultural Affairs, Belgium
Funding country
  • EU
  • Belgium
Funding type
  • government

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Cite this study

MLA

Windels, P, I Taverniers, A Depicker, E Van Bockstaele, M De Loose. "Characterisation of the Roundup Ready soybean insert." European Food Research and Technology 213.2 (2001): 107-112. Web. 24 Sep. 2018.

APA

Windels, P., Taverniers, I., Depicker, A., Van Bockstaele, E., De Loose, M. (2001). Characterisation of the Roundup Ready soybean insert. European Food Research and Technology, 213(2), 107-112. doi:10.1007/s002170100336

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