There are currently 402 studies in the GENERA database.

Advanced Search

General Information

Document type
  • Peer-reviewed journal article
GE organism
  • plum
GE trait
  • disease resistance
Country
  • Canada

Results

Efficacy
  • positive effect

Silencing of the host factor eIF(iso)4E gene confers plum pox virus resistance in plum Open Access

Wang, X; Kohalmi, SE; Svircev, A; Wang, A; Sanfaçon, H; Tian, L
PLOS ONE. 2013 January. 8(1):e50627

Link to full text (open access, freely available)

PMID: 23382802 DOI: 10.1371/journal.pone.0050627 ISSN: 1932-6203

Abstract

Plum pox virus (PPV) causes the most economically-devastating viral disease in Prunus species. Unfortunately, few natural resistance genes are available for the control of PPV. Recessive resistance to some potyviruses is associated with mutations of eukaryotic translation initiation factor 4E (eIF4E) or its isoform eIF(iso)4E. In this study, we used an RNA silencing approach to manipulate the expression of eIF4E and eIF(iso)4E towards the development of PPV resistance in Prunus species. The eIF4E and eIF(iso)4E genes were cloned from plum (Prunus domestica L.). The sequence identity between plum eIF4E and eIF(iso)4E coding sequences is 60.4% at the nucleotide level and 52.1% at the amino acid level. Quantitative real-time RT-PCR analysis showed that these two genes have a similar expression pattern in different tissues. Transgenes allowing the production of hairpin RNAs of plum eIF4E or eIF(iso)4E were introduced into plum via Agrobacterium-mediated transformation. Gene expression analysis confirmed specific reduced expression of eIF4E or eIF(iso)4E in the transgenic lines and this was associated with the accumulation of siRNAs. Transgenic plants were challenged with PPV-D strain and resistance was evaluated by measuring the concentration of viral RNA. Eighty-two percent of the eIF(iso)4E silenced transgenic plants were resistant to PPV, while eIF4E silenced transgenic plants did not show PPV resistance. Physical interaction between PPV-VPg and plum eIF(iso)4E was confirmed. In contrast, no PPV-VPg/eIF4E interaction was observed. These results indicate that eIF(iso)4E is involved in PPV infection in plum, and that silencing of eIF(iso)4E expression can lead to PPV resistance in Prunus species.

Keywords

plum pox virus; resistance; prunus; RNA silencing; RNAi

Funding

Funding source
  • Not reported
Funding country
  • Not reported
Funding type
  • Not reported

Links to outside analysis of this resource

Please contact us if you know of an independent summary or analysis of this resource.

Cite this study

MLA

Wang, X, SE Kohalmi, A Svircev, A Wang, H Sanfaçon, L Tian. "Silencing of the host factor eIF(iso)4E gene confers plum pox virus resistance in plum." PLOS ONE 8.1 (2013): e50627. Web. 24 Sep. 2018.

APA

Wang, X., Kohalmi, SE., Svircev, A., Wang, A., Sanfaçon, H., & Tian, L. (2013). Silencing of the host factor eIF(iso)4E gene confers plum pox virus resistance in plum. PLOS ONE, 8(1), e50627. doi:10.1371/journal.pone.0050627

Please verify citations before use, citations are automatically generated based on information stored within the GENERA database and therefore may or may not be correct.