There are currently 402 studies in the GENERA database.

Advanced Search

General Information

Document type
  • Peer-reviewed journal article
GE organism
  • rice
GE trait
  • quality
Country
  • Japan

Results

Efficacy
  • positive effect
Equivalence
  • no effect

High-level tryptophan accumulation in seeds of transgenic rice and its limited effects on agronomic traits and seed metabolite profile Open Access

Wakasa, K; Hasegawa, H; Nemoto, H; Matsuda, F; Miyazawa, H; Tozawa, Y; Morino, K; Komatsu, A; Yamada, T; Terakawa, T; Miyagawa, H
Journal of Experimental Botany. 2006 August. 57(12):3069-3078

Link to full text (open access, freely available)

PMID: 16908506 DOI: 10.1093/jxb/erl068 ISSN: 0022-0957

Abstract

Metabolic manipulation of plants to improve their nutritional quality is an important goal of plant biotechnology. Expression in rice (Oryza sativa L.) of a transgene (OASA1D) encoding a feedback-insensitive alpha subunit of rice anthranilate synthase results in the accumulation of tryptophan (Trp) in calli and leaves. It is shown here that the amount of free Trp in the seeds of such plants is increased by about two orders of magnitude compared with that in the seeds of wild-type plants. The total Trp content in the seeds of the transgenic plants was also increased. Two homozygous lines, HW1 and HW5, of OASA1D transgenic rice were generated for characterization of agronomic traits and aromatic metabolite profiling of seeds. The marked overproduction of Trp was stable in these lines under field conditions, although spikelet fertility and yield, as well as seed germination ability, were reduced compared with the wild type. These differences in agronomic traits were small, however, in HW5. In spite of the high Trp content in the seeds of the HW lines, metabolic profiling revealed no substantial changes in the amounts of other phenolic compounds. The amount of indole acetic acid was increased about 2-fold in the seeds of the transgenic lines. The establishment and characterization of these OASA1D transgenic lines have thus demonstrated the feasibility of increasing the Trp content in the seeds of rice (or of other crops) as a means of improving its nutritional value for human consumption or animal feed.

Keywords

Amino acids, anthranilate synthase, AS, non-containment greenhouse, IAA, isolated field, 5MT, OASA1D, Oryza sativa

Funding

Funding source
  • Japan Science and Technology Agency
  • National Agriculture and Bio-oriented Research Organization.
Funding country
  • Japan
Funding type
  • government

Links to outside analysis of this resource

Please contact us if you know of an independent summary or analysis of this resource.

Cite this study

MLA

Wakasa, K, H Hasegawa, H Nemoto, F Matsuda, H Miyazawa, Y Tozawa, K Morino, A Komatsu, T Yamada, T Terakawa, H Miyagawa. "High-level tryptophan accumulation in seeds of transgenic rice and its limited effects on agronomic traits and seed metabolite profile." Journal of Experimental Botany 57.12 (2006): 3069-3078. Web. 24 Sep. 2018.

APA

Wakasa, K., Hasegawa, H., Nemoto, H., Matsuda, F., Miyazawa, H., Tozawa, Y., Morino, K., Komatsu, A., Yamada, T., Terakawa, T., & Miyagawa, H. (2006). High-level tryptophan accumulation in seeds of transgenic rice and its limited effects on agronomic traits and seed metabolite profile. Journal of Experimental Botany, 57(12), 3069-3078. doi:10.1093/jxb/erl068

Please verify citations before use, citations are automatically generated based on information stored within the GENERA database and therefore may or may not be correct.