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General Information

Document type
  • Journal Article
GE organism
  • Rice
GE trait
  • pharmaceutical
Country
  • Japan

Results

Equivalence
  • no effect

Growth characteristics of rice cell genetically modified for recombinant human α-antitrypsin production

Terashima, Masaaki; Hashikawa, Naohiro; Hattori, Masayas; Yoshida, Hiroyuki
Biochemical Engineering Journal. 2002 November. 12(2):155-160

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DOI: 10.1016/S1369-703X(02)00064-5 ISSN: 1369-703X

Abstract

Fundamental growth characteristics of non-transformed rice cell and genetically modified rice cell have been studied to evaluate their potential as a host system for recombinant protein production. Specific growth rate in 5l fermenter was about 0.41 liter per day for both the non-transformed cell and the genetically modified cell with initial sucrose concentration 30g/l. Although the specific growth rate of the genetically modified cell was almost the same as that of the non-transformed cell, period of log-phase was shorter than that of the non-transformed cell. While growth yield of the non-transformed rice cell was 0.38 for initial sucrose concentration of 30g/l, those values were larger than 0.5 for initial sucrose concentrations 10 and 15g/l. The growth yield of the genetically modified cell was 0.19 for initial sucrose concentration of 30g/l. Dry cell weight of the genetically modified rice cell reached 12g/l in 5l fermenter. It was found that kinetin concentration decreased rapidly, and ammonia was produced in culture medium during cell culture.

Keywords

Recombinant protein; Rice cell; Cell culture; Growth characteristics; Transgenic plant

Funding

Funding source
  • Ministry of Education, Culture, Sports, Science and Technology
Funding country
  • Japan
Funding type
  • government

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Cite this study

MLA

Terashima, Masaaki, Naohiro Hashikawa, Masayas Hattori, Hiroyuki Yoshida. "Growth characteristics of rice cell genetically modified for recombinant human α-antitrypsin production." Biochemical Engineering Journal 12.2 (2002): 155-160. Web. 13 Dec. 2018.

APA

Terashima, Masaaki., Hashikawa, Naohiro., Hattori, Masayas., & Yoshida, Hiroyuki. (2002). Growth characteristics of rice cell genetically modified for recombinant human α-antitrypsin production. Biochemical Engineering Journal, 12(2), 155-160. doi:10.1016/S1369-703X(02)00064-5

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