Terashima, Masaaki; Hashikawa, Naohiro; Hattori, Masayas; Yoshida, Hiroyuki
Biochemical Engineering Journal. 2002 November. 12(2):155-160
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DOI: 10.1016/S1369-703X(02)00064-5 ISSN: 1369-703X
Fundamental growth characteristics of non-transformed rice cell and genetically modified rice cell have been studied to evaluate their potential as a host system for recombinant protein production. Specific growth rate in 5l fermenter was about 0.41 liter per day for both the non-transformed cell and the genetically modified cell with initial sucrose concentration 30g/l. Although the specific growth rate of the genetically modified cell was almost the same as that of the non-transformed cell, period of log-phase was shorter than that of the non-transformed cell. While growth yield of the non-transformed rice cell was 0.38 for initial sucrose concentration of 30g/l, those values were larger than 0.5 for initial sucrose concentrations 10 and 15g/l. The growth yield of the genetically modified cell was 0.19 for initial sucrose concentration of 30g/l. Dry cell weight of the genetically modified rice cell reached 12g/l in 5l fermenter. It was found that kinetin concentration decreased rapidly, and ammonia was produced in culture medium during cell culture.
Terashima, Masaaki, Naohiro Hashikawa, Masayas Hattori, Hiroyuki Yoshida. "Growth characteristics of rice cell genetically modified for recombinant human α-antitrypsin production." Biochemical Engineering Journal 12.2 (2002): 155-160. Web. 18 Feb. 2019.
Terashima, Masaaki., Hashikawa, Naohiro., Hattori, Masayas., & Yoshida, Hiroyuki. (2002). Growth characteristics of rice cell genetically modified for recombinant human α-antitrypsin production. Biochemical Engineering Journal, 12(2), 155-160. doi:10.1016/S1369-703X(02)00064-5
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