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General Information

Document type
  • Peer-reviewed journal article
GE organism
  • rice
GE trait
  • agronomic properties
Country
  • Japan

Results

Equivalence
  • mixed

High throughput metabolome and proteome analysis of transgenic rice plants (Oryza sativa L.) Open Access

Takahashi H, Hotta Y, Hayashi M, Kawai-Yamada M, Komatsu S, Uchimiya H
Plant Biotechnology. 2005 22(1):47-50

Link to full text (open access, freely available)

DOI: 10.5511/plantbiotechnology.22.47 ISSN: 1342-4580

Abstract

We profiled metabolic patterns of transgenic rice organs and tissue by Fourier-transform ion cyclotron mass spectrometry (FT-MS) to reveal effects of the over-expression of YK1 possessing high homology with maize HC-toxin reductase gene. Comparison of metabolic patterns revealed that compositions of organ- or tissue-specific metabolites were not significantly varied between the control and the YK1 rice, where expression levels of some metabolites were altered. Proteome analysis of cultured cells over-expressing YK1 showed the up-regulation of several stress-related proteins such as osmotin-like protein and osr40c1. Thus, alteration of metabolites as well as proteins may contribute to multiple stress tolerances in transgenic YK1 rice.

Keywords

Metabolome, proteome, Fourier-transform ion cyclotron mass spectrometry (FT-MS), HC-toxin reductase like rice gene (YK1), rice (Oryza sativa L.)

Funding

Funding source
  • Japan Society for the Promotion of Science
Funding country
  • Japan
Funding type
  • government

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Cite this study

MLA

Takahashi H, Hotta Y, Hayashi M, Kawai-Yamada M, Komatsu S, Uchimiya H. "High throughput metabolome and proteome analysis of transgenic rice plants (Oryza sativa L.)." Plant Biotechnology 22.1 (2005): 47-50. Web. 24 Sep. 2018.

APA

Takahashi H, Hotta Y, Hayashi M, Kawai-Yamada M, Komatsu S, Uchimiya H. (2005). High throughput metabolome and proteome analysis of transgenic rice plants (Oryza sativa L.). Plant Biotechnology, 22(1), 47-50. doi:10.5511/plantbiotechnology.22.47

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