Takahashi H, Hotta Y, Hayashi M, Kawai-Yamada M, Komatsu S, Uchimiya H
Plant Biotechnology. 2005 22(1):47-50
Link to full text (open access, freely available)
DOI: 10.5511/plantbiotechnology.22.47 ISSN: 1342-4580
We profiled metabolic patterns of transgenic rice organs and tissue by Fourier-transform ion cyclotron mass spectrometry (FT-MS) to reveal effects of the over-expression of YK1 possessing high homology with maize HC-toxin reductase gene. Comparison of metabolic patterns revealed that compositions of organ- or tissue-specific metabolites were not significantly varied between the control and the YK1 rice, where expression levels of some metabolites were altered. Proteome analysis of cultured cells over-expressing YK1 showed the up-regulation of several stress-related proteins such as osmotin-like protein and osr40c1. Thus, alteration of metabolites as well as proteins may contribute to multiple stress tolerances in transgenic YK1 rice.
Takahashi H, Hotta Y, Hayashi M, Kawai-Yamada M, Komatsu S, Uchimiya H. "High throughput metabolome and proteome analysis of transgenic rice plants (Oryza sativa L.)." Plant Biotechnology 22.1 (2005): 47-50. Web. 18 Jul. 2018.
Takahashi H, Hotta Y, Hayashi M, Kawai-Yamada M, Komatsu S, Uchimiya H. (2005). High throughput metabolome and proteome analysis of transgenic rice plants (Oryza sativa L.). Plant Biotechnology, 22(1), 47-50. doi:10.5511/plantbiotechnology.22.47
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