There are currently 402 studies in the GENERA database.

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General Information

Document type
  • Peer-reviewed journal article
GE organism
  • Arabidopsis
  • oilseed rape
GE trait
  • industrial product
Country
  • USA

Results

Efficacy
  • positive effect

Metabolic engineering of Arabidopsis and Brassica for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer production

Slater, S; Mitsky, TA; Houmiel, KL; Hao, M; Reiser, SE; Taylor, NB; Tran, M; Valentin, HE; Rodriguez, DJ; Stone, DA; Padgette, SR; Kishore, G; Gruys, KJ
Nature Biotechnology. 1999 October. 17(10):1011-1016

Link to full text (journal may charge for access)

PMID: 10504704 DOI: 10.1038/13711 ISSN: 1087-0156

Abstract

Poly(hydroxyalkanoates) are natural polymers with thermoplastic properties. One polymer of this class with commercial applicability, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) can be produced by bacterial fermentation, but the process is not economically competitive with polymer production from petrochemicals. Poly(hydroxyalkanoate) production in green plants promises much lower costs, but producing copolymer with the appropriate monomer composition is problematic. In this study, we have engineered Arabidopsis and Brassica to produce PHBV in leaves and seeds, respectively, by redirecting the metabolic flow of intermediates from fatty acid and amino acid biosynthesis. We present a pathway for the biosynthesis of PHBV in plant plastids, and also report copolymer production, metabolic intermediate analyses, and pathway dynamics.

Keywords

poly(hydroxyalkanoate), biodegradable polymer, green chemistry, PHA copolymer

Funding

Funding source
  • Monsanto
Funding country
  • United States
Funding type
  • industry: same

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Cite this study

MLA

Slater, S, TA Mitsky, KL Houmiel, M Hao, SE Reiser, NB Taylor, M Tran, HE Valentin, DJ Rodriguez, DA Stone, SR Padgette, G Kishore, KJ Gruys. "Metabolic engineering of Arabidopsis and Brassica for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer production." Nature Biotechnology 17.10 (1999): 1011-1016. Web. 13 Dec. 2018.

APA

Slater, S., Mitsky, TA., Houmiel, KL., Hao, M., Reiser, SE., Taylor, NB., Tran, M., Valentin, HE., Rodriguez, DJ., Stone, DA., Padgette, SR., Kishore, G., & Gruys, KJ. (1999). Metabolic engineering of Arabidopsis and Brassica for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymer production. Nature Biotechnology, 17(10), 1011-1016. doi:10.1038/13711

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