There are currently 402 studies in the GENERA database.

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General Information

Document type
  • Peer-reviewed journal article
GE organism
  • maize
GE trait
  • quality
Country
  • Mexico

Results

Safety for consumption
  • no effect

Safety assessment by in vitro digestibility and allergenicity of genetically modified maize with an amaranth 11S globulin

Sinagawa-García, SR; Rascón-Cruz, Q; Valdez-Ortiz, A; Medina-Godoy, S; Escobar-Gutiérrez, A; Paredes-López, O.
Journal of Agricultural and Food Chemistry. 2004 April. 52(9):2709–2714

Link to full text (journal may charge for access)

PMID: 15113180 DOI: 10.1021/jf035487k

Abstract

Prospective testing for allergenicity of proteins obtained from sources with no prior history of causing allergy has been difficult to perform. Thus, the objective of this work was to assess the food safety of genetically modified maize with an amaranth globulin protein termed amarantin. Transgenic maize lines evaluated showed, in relation to nontransgenic, 4-35% more protein and 0-44% higher contents of specific essential amino acids. Individual sequence analysis with known amino acid sequences, reported as allergens, showed that none of these IgE elicitors were identified in amarantin. Amarantin was digested within the first 15 min by Simulated Gastric Fluid treatment as observed by Western blot. Expressed amarantin did not induce important levels of specific IgE antibodies in BALB/c mice, as analyzed by ELISA. We conclude that the transgenic maize with amarantin is not an important allergenicity inducer, just as nontransgenic maize.

Keywords

Food safety; transgenic maize; Amaranthus; amarantin; in vitro digestibility; allergenicity; animal model

Funding

Funding source
  • National Council of Science and Technology, Mexico (CONACyT-Mexico)
  • Council of Science and Technology of the State of Guanajuato (CONCyTEG)
Funding country
  • Mexico
Funding type
  • government

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Cite this study

MLA

Sinagawa-García, SR, Q Rascón-Cruz, A Valdez-Ortiz, S Medina-Godoy, A Escobar-Gutiérrez, O. Paredes-López. "Safety assessment by in vitro digestibility and allergenicity of genetically modified maize with an amaranth 11S globulin." Journal of Agricultural and Food Chemistry 52.9 (2004): 2709–2714. Web. 13 Dec. 2018.

APA

Sinagawa-García, SR., Rascón-Cruz, Q., Valdez-Ortiz, A., Medina-Godoy, S., Escobar-Gutiérrez, A., Paredes-López, O.. (2004). Safety assessment by in vitro digestibility and allergenicity of genetically modified maize with an amaranth 11S globulin. Journal of Agricultural and Food Chemistry, 52(9), 2709–2714. doi:10.1021/jf035487k

Please verify citations before use, citations are automatically generated based on information stored within the GENERA database and therefore may or may not be correct.