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General Information

Document type
  • Peer-reviewed journal article
GE organism
  • pea
GE trait
  • insect resistance
Country
  • Australia, USA

Results

Safety for consumption
  • negative effect

Transgenic expression of bean alpha-amylase inhibitor in peas results in altered structure and immunogenicity

Prescott, VE; Campbell, PM; Moore, A; Mattes, J; Rothenberg, ME; Foster, PS; Higgins, TJ; Hogan, SP
Journal of Agricultural and Food Chemistry. 2005 October. 53(23):9023–9030

Link to full text (journal may charge for access)

PMID: 16277398 DOI: 10.1021/jf050594v

Abstract

The development of modern gene technologies allows for the expression of recombinant proteins in non-native hosts. Diversity in translational and post-translational modification pathways between species could potentially lead to discrete changes in the molecular architecture of the expressed protein and subsequent cellular function and antigenicity. Here, we show that transgenic expression of a plant protein (alpha-amylase inhibitor-1 from the common bean (Phaseolus vulgaris L. cv. Tendergreen)) in a non-native host (transgenic pea (Pisum sativum L.)) led to the synthesis of a structurally modified form of this inhibitor. Employing models of inflammation, we demonstrated in mice that consumption of the modified alphaAI and not the native form predisposed to antigen-specific CD4+ Th2-type inflammation. Furthermore, consumption of the modified alphaAI concurrently with other heterogeneous proteins promoted immunological cross priming, which then elicited specific immunoreactivity of these proteins. Thus, transgenic expression of non-native proteins in plants may lead to the synthesis of structural variants possessing altered immunogenicity.

Keywords

α-Amylase inhibitor; transgenic plant; animal model; Th2 inflammation; mass spectrophotometry

Funding

Funding source
  • National Health Medical Research Council
Funding country
  • Australia
Funding type
  • government

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Cite this study

MLA

Prescott, VE, PM Campbell, A Moore, J Mattes, ME Rothenberg, PS Foster, TJ Higgins, SP Hogan. "Transgenic expression of bean alpha-amylase inhibitor in peas results in altered structure and immunogenicity." Journal of Agricultural and Food Chemistry 53.23 (2005): 9023–9030. Web. 21 Nov. 2018.

APA

Prescott, VE., Campbell, PM., Moore, A., Mattes, J., Rothenberg, ME., Foster, PS., Higgins, TJ., & Hogan, SP. (2005). Transgenic expression of bean alpha-amylase inhibitor in peas results in altered structure and immunogenicity. Journal of Agricultural and Food Chemistry, 53(23), 9023–9030. doi:10.1021/jf050594v

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