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General Information

Document type
  • Peer-reviewed journal article
Study Type
  • bioinformatics
  • allergenicity assessment
GE organism
  • Multiple
GE trait
  • disease resistance
  • agronomic properties
Country
  • India

Results

Safety for consumption
  • mixed

In silico assessment of the potential allergenicity of transgenes used for the development of GM food crops

Mishra, A; Gaur, SN; Singh, BP; Arora, N
Food and Chemical Toxicology. 2012 May. 50(5):1334–1339

Link to full text (journal may charge for access)

PMID: 22361217 DOI: 10.1016/j.fct.2012.02.005 ISSN: 0278-6915

Abstract

Genetically modified (GM) crops require allergenicity and toxicity assessment of the novel protein(s) to ensure complete safety to the consumers. These assessments are performed in accordance with the guidelines proposed by Codex (2003) and ICMR (2008). The guidelines recommend sequence homology analysis as a preliminary step towards allergenicity prediction, later in vitro experiments may be performed to confirm allergenicity. In the present study, an in silico approach is employed to evaluate the allergenic potential of six transgenes routinely used for the development of GM food crops. Among the genes studied, manganese superoxide dismutase (MnSOD) and osmotin shares greater than 90% identity with Hev b 10 and Cap a 1w, respectively. Chitinase shares greater than 70% identity with allergens namely Pers a 1 and Hev b 11, and fungal chitinase showed significant IgE binding with 7 of 75 patients' sera positive to different food extracts. Glucanases (alfalfa, wheat) and glycine betaine aldehyde dehydrogenase gene share 50% homology with allergens like - Ole e 9, Cla h 10 and Alt a 10. The results demonstrate the allergenic potential of six genes and can serve as a guide for selection of transgenes to develop GM crops.

Keywords

Food allergy; Genetically modified foods; Transgenes; Sequence homology; Safety assessments

Funding

Funding source
  • Not reported
Funding country
  • Not reported
Funding type
  • Not reported

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Cite this study

MLA

Mishra, A, SN Gaur, BP Singh, N Arora. "In silico assessment of the potential allergenicity of transgenes used for the development of GM food crops." Food and Chemical Toxicology 50.5 (2012): 1334–1339. Web. 11 Dec. 2018.

APA

Mishra, A., Gaur, SN., Singh, BP., & Arora, N. (2012). In silico assessment of the potential allergenicity of transgenes used for the development of GM food crops. Food and Chemical Toxicology, 50(5), 1334–1339. doi:10.1016/j.fct.2012.02.005

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