There are currently 402 studies in the GENERA database.

Advanced Search

General Information

Document type
  • Peer-reviewed journal article
GE organism
  • tomato
GE trait
  • quality
  • Germany
  • Spain


  • positive effect
Safety for consumption
  • positive effect

Skin prick tests reveal stable and heritable reduction of allergenic potency of gene-silenced tomato fruits Open Access

Lorenz, Y; Enrique, E; Lequynh, L; Fötisch, K; Retzek, M; Biemelt, S; Sonnewald, U; Vieths, S; Scheurer, S
The Journal of Allergy and Clinical Immunology. 2006 September. 118(3):711-718

Link to full text (open access, freely available)

PMID: 16950292 DOI: 10.1016/j.jaci.2006.05.014 ISSN: 0091-6749


BACKGROUND: Today, for patients with food allergy, the only possibility to prevent allergic reactions is avoidance of the allergenic food. Genetic engineering of hypoallergenic plants by means of RNA interference (RNAi) could be an approach to improve the quality of life of subjects with food allergy. OBJECTIVES: We sought to achieve stable inhibition of expression of the allergenic nonspecific lipid transfer protein Lyc e 3 in tomato and to analyze the reduction of allergenicity in vitro by using histamine release assays and in vivo by using skin prick tests with transgenic tomato fruits. METHODS: Gene silencing was performed by means of RNAi and monitored by using Western blotting with nonspecific lipid transfer protein-specific antibodies and sera from patients with tomato allergy. Dose-dependent basophil histamine release assays, prick-to-prick skin testing, and determination of endogenous histamine content were performed with fruits harvested from plants of the first and second generation to assess the allergenic potency compared with that of wild-type fruits. RESULTS: We demonstrated that silencing of Lyc e 3 by means of RNAi contributes to reduced skin reactivity and is passed on to the next generation of fruits. A significant reduction of allergenic potency was determined in vitro and confirmed by using skin prick tests. CONCLUSION: Taken together, these results indicate that RNAi technology is an effective tool to generate foods with reduced allergenicity. CLINICAL IMPLICATIONS: Allergen-reduced plant foods might allow reduction of dietary restrictions for patients allergic to panallergen families.


Gene silencing, RNA interference, food allergy, tomato, Lyc e 3, allergenic potency, hypoallergenic, genetically modified food


Funding source
  • German Research Foundation
Funding country
  • Germany
Funding type
  • government

Links to outside analysis of this resource

Please contact us if you know of an independent summary or analysis of this resource.

Cite this study


Lorenz, Y, E Enrique, L Lequynh, K Fötisch, M Retzek, S Biemelt, U Sonnewald, S Vieths, S Scheurer. "Skin prick tests reveal stable and heritable reduction of allergenic potency of gene-silenced tomato fruits." The Journal of Allergy and Clinical Immunology 118.3 (2006): 711-718. Web. 28 May. 2024.


Lorenz, Y., Enrique, E., Lequynh, L., Fötisch, K., Retzek, M., Biemelt, S., Sonnewald, U., Vieths, S., & Scheurer, S. (2006). Skin prick tests reveal stable and heritable reduction of allergenic potency of gene-silenced tomato fruits. The Journal of Allergy and Clinical Immunology, 118(3), 711-718. doi:10.1016/j.jaci.2006.05.014

Please verify citations before use, citations are automatically generated based on information stored within the GENERA database and therefore may or may not be correct.