There are currently 402 studies in the GENERA database.

Advanced Search

General Information

Document type
  • Peer-reviewed journal article
GE organism
  • broccoli
GE trait
  • agronomic properties
  • Taiwan


Safety for consumption
  • no effect

Nonadverse effects on allergenicity of isopentenyltransferase-transformed broccoli Open Access

Liao, EC; Chen, JT; Chao, ML; Yu, SC; Chang, CY; Chu, WS; Tsai, JJ
Journal of Investigational Allergology and Clinical Immunology. 2013 23(2):112-119

Link to full text (open access, freely available)

PMID: 23654078


BACKGROUND: Genetically modified organisms (GMOs) provide modern agriculture with improvements in efficiency and the benefits of enhanced food production; however, the potential impact of GMOs on human health has not yet been clarified. OBJECTIVE: To investigate the allergenicity of isopentenyltransferase (ipt)-transformed broccoli compared with non-GM broccoli. METHODS: Sera from allergic individuals were used to identify the allergenicity of GM and non-GM broccoli. Immunoglobulin (Ig) binding of different lines of GM and non-GM broccoli was identified using immunoblotting, enzyme-linked immunosorbent assay, and the histamin release assay. RESULTS: Positive reactions to broccoli (Brassica Oleracea) were observed in 7.02% of individuals. Specific IgE to broccoli and total IgE fro allergic individuals were well correlated. The different tests performed showed no significant differences in the allergenicity of conventionally raised and GM broccoli, indicating the absence of unexpected effects on allergenicity in ipt-transformed plants. Using Western blot analysis we detected heterogeneous IgE-reactive allergenic components in broccoli-allergic sera, but no significant differences between GM an non-GM broccoli were observed in serum from the same patients. CONCLUSIONS: Our study demonstrates that there are no differences between GM (ipt-transformed) broccoli and non-GM broccoli, as determined by specific IgE in sera from broccoli-allergic patients. This indicates that there were no unexpected effects on allergenicity in this GM broccoli.


Genetically modified organisms. Isopentenyltransferase gene. Broccoli allergenicity.


Funding source
  • Food and Drug Administration
  • Department of Health, Executive Yuan, Taiwan R.O.C.
Funding country
  • United States
  • Taiwan
Funding type
  • government

Links to outside analysis of this resource

Please contact us if you know of an independent summary or analysis of this resource.

Cite this study


Liao, EC, JT Chen, ML Chao, SC Yu, CY Chang, WS Chu, JJ Tsai. "Nonadverse effects on allergenicity of isopentenyltransferase-transformed broccoli." Journal of Investigational Allergology and Clinical Immunology 23.2 (2013): 112-119. Web. 24 Apr. 2024.


Liao, EC., Chen, JT., Chao, ML., Yu, SC., Chang, CY., Chu, WS., & Tsai, JJ. (2013). Nonadverse effects on allergenicity of isopentenyltransferase-transformed broccoli. Journal of Investigational Allergology and Clinical Immunology, 23(2), 112-119.

Please verify citations before use, citations are automatically generated based on information stored within the GENERA database and therefore may or may not be correct.