There are currently 402 studies in the GENERA database.

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General Information

Document type
  • Peer-reviewed journal article
Study Type
  • allergenicity test
GE organism
  • maize
GE trait
  • insect resistance
Country
  • Portugal

Results

Equivalence
  • no effect
Safety for consumption
  • no effect

Characterization of maize allergens - MON810 vs. its non-transgenic counterpart.

Fonseca, C; Planchon, S; Renaut, J; Oliveira, MM; Batista, R
Journal of Proteomics. 2012 April. 75(7):2027–2037

Link to full text (journal may charge for access)

PMID: 22270010 DOI: 10.1016/j.jprot.2012.01.005 ISSN: 1874-3919

Abstract

One of the main concerns about genetically modified foods and their potential impacts on human health is that the introduction of a new/ altered gene may putatively alter the expression of others, namely endogenous allergens. We intended to evaluate, and to compare, using quantitative real time RT-PCR technique, the expression of 5 already known maize allergens (Zea m14, Zea m25, Zea m27kD, 50kD Zein and trypsin inhibitor) in MON 810 vs. its non-transgenic counterpart, throughout seed development (10, 16 and 23days after pollination). We have shown that none of the tested allergen genes presented differential expression, with statistic significance, along all tested seed development stages, in MON810 vs. its conventional counterpart. We have also used bidimensional gel electrophoresis followed by Western blotting with plasma from two maize allergic subjects to characterize their immunologic responses against MON 810 vs. its non-transgenic control. Immunoreactive spots were characterized by MS. We have identified fourteen new IgE-binding proteins present in both transgenic and non-transgenic maize.

Keywords

Maize allergy; Genetically modified food safety; Allergen identification; 2D-gel electrophoresis; Western blot; MON810

Funding

Funding source
  • Not reported
Funding country
  • Not reported
Funding type
  • Not reported

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Cite this study

MLA

Fonseca, C, S Planchon, J Renaut, MM Oliveira, R Batista. "Characterization of maize allergens - MON810 vs. its non-transgenic counterpart.." Journal of Proteomics 75.7 (2012): 2027–2037. Web. 13 Dec. 2018.

APA

Fonseca, C., Planchon, S., Renaut, J., Oliveira, MM., & Batista, R. (2012). Characterization of maize allergens - MON810 vs. its non-transgenic counterpart.. Journal of Proteomics, 75(7), 2027–2037. doi:10.1016/j.jprot.2012.01.005

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