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General Information

Document type
  • Peer-reviewed journal article
GE organism
  • potato
GE trait
  • insect resistance
  • USA, Egypt


  • positive effect
  • no effect

Development of Bt-cry5 Insect-resistant Potato Lines 'Spunta-G2' and 'Spunta-G3' Open Access

Douches, DS; Li, W; Zarka, K; Coombs, J; Pett, W; Grafius, E; El-Nasr, T
HortScience. 2002 December. 37(7):1103-1107

Link to full text (open access, freely available)

ISSN: 2327-9834


The potato tuber moth (Phthorimaea operculella Zeller) is the primary insect pest of cultivated potato (Solanum tuberosum L.) in tropical and subtropical regions, causing both foliar and tuber damage. In contrast, the Colorado potato beetle (Leptinotarsa decemlineata Say) is the most important insect pest in the northern potato production latitudes. The codon-modified Bacillus thuringiensis Bt-cry5 gene (revised nomenclature cry1IaI), specifically toxic to Lepidoptera and Coleoptera, was transformed into cultivar Spunta using an Agrobacterium vector to provide resistance to both potato tuber moth and Colorado potato beetle. The Bt-cry5 gene was placed downstream from the constitutive CaMV35S promoter. Two transgenic 'Spunta' clones, G2 and G3, produced high levels of mortality in first instars of potato tuber moth in detached-leaf bioassays (80% to 83% mortality), laboratory tuber tests (100% mortality), and field trials in Egypt (99% to 100% undamaged tubers). Reduced feeding by Colorado potato beetle first instars was also observed in detached-leaf bioassays (80% to 90% reduction). Field trials in the United States demonstrated that the horticultural performance of the two transgenic lines was comparable to 'Spunta'. These Bt-cry5 transgenic potato plants with high potato tuber moth resistance have value in integrated pest management programs.


Bacillus thuringiensis, transgenic plant, Solanum tuberosum, potato tuber moth, Phthorimaea operculella, Colorado potato beetle, Leptinotarsa decemlineata


Funding source
  • Michigan Agriculture Experiment Station
Funding country
  • United States
Funding type
  • government

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Cite this study


Douches, DS, W Li, K Zarka, J Coombs, W Pett, E Grafius, T El-Nasr. "Development of Bt-cry5 Insect-resistant Potato Lines 'Spunta-G2' and 'Spunta-G3'." HortScience 37.7 (2002): 1103-1107. Web. 7 Dec. 2023.


Douches, DS., Li, W., Zarka, K., Coombs, J., Pett, W., Grafius, E., El-Nasr, T. (2002). Development of Bt-cry5 Insect-resistant Potato Lines 'Spunta-G2' and 'Spunta-G3'. HortScience, 37(7), 1103-1107.

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