There are currently 402 studies in the GENERA database.

Advanced Search

General Information

Document type
  • Peer-reviewed journal article
GE organism
  • maize
GE trait
  • quality
Country
  • USA

Results

Efficacy
  • positive effect

Transgenic maize endosperm containing a milk protein has improved amino acid balance

Bicar, EH; Woodman-Clikeman, W; Sangtong, V; Peterson, JM; Yang, SS; Lee, M; Scott, MP
Transgenic Research. 2008 February. 17(1):59-71

Link to full text (journal may charge for access)

PMID: 17387628 DOI: 10.1007/s11248-007-9081-3 ISSN: 1573-9368

Abstract

In order to meet the protein nutrition needs of the world population, greater reliance on plant protein sources will become necessary. The amino acid balance of most plant protein sources does not match the nutritional requirements of monogastric animals, limiting their nutritional value. In cereals, the essential amino acid lysine is deficient. Maize is a major component of human and animal diets worldwide and especially where sources of plant protein are in critical need such as sub-Saharan Africa. To improve the amino acid balance of maize, we developed transgenic maize lines that produce the milk protein alpha-lactalbumin in the endosperm. Lines in which the transgene was inherited as a single dominant genetic locus were identified. Sibling kernels with or without the transgene were compared to determine the effect of the transgene on kernel traits in lines selected for their high content of alpha-lactalbumin. Total protein content in endosperm from transgene positive kernels was not significantly different from total protein content in endosperm from transgene negative kernels in three out of four comparisons, whereas the lysine content of the lines examined was 29-47% greater in endosperm from transgene positive kernels. The content of some other amino acids was changed to a lesser extent. Taken together, these changes resulted in the transgenic endosperms having an improved amino acid balance relative to non-transgenic endosperms produced on the same ear. Kernel appearance, weight, density and zein content did not exhibit substantial differences in kernels expressing the transgene when compared to non-expressing siblings. Assessment of the antigenicity and impacts on animal health will be required in order to determine the overall value of this technology.

Keywords

Transgene, Maize, α-Lactalbumin, Lysine, Nutrition, Grain

Funding

Funding source
  • Iowa Corn Promotion Board
  • Raymond F. Baker Center for Plant Breeding
  • Pioneer Hibred International
Funding country
  • United States
Funding type
  • industry: same
  • NGO: same industry aligned
  • NGO: independent

Links to outside analysis of this resource

Please contact us if you know of an independent summary or analysis of this resource.

Cite this study

MLA

Bicar, EH, W Woodman-Clikeman, V Sangtong, JM Peterson, SS Yang, M Lee, MP Scott. "Transgenic maize endosperm containing a milk protein has improved amino acid balance." Transgenic Research 17.1 (2008): 59-71. Web. 21 Nov. 2018.

APA

Bicar, EH., Woodman-Clikeman, W., Sangtong, V., Peterson, JM., Yang, SS., Lee, M., & Scott, MP. (2008). Transgenic maize endosperm containing a milk protein has improved amino acid balance. Transgenic Research, 17(1), 59-71. doi:10.1007/s11248-007-9081-3

Please verify citations before use, citations are automatically generated based on information stored within the GENERA database and therefore may or may not be correct.