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General Information

Document type
  • Peer-reviewed journal article
GE organism
  • ryegrass
GE trait
  • quality


  • positive effect

Knocking out expression of plant allergen genes

Bhalla, PL; Singh, MB
Methods. 2004 March. 32(3):340-5

Link to full text (journal may charge for access)

PMID: 14962769 DOI: 10.1016/j.ymeth.2003.08.011 ISSN: 1046-2023


Pollen of many grasses, trees, and weeds are the source of inhalant allergic proteins while various other plant products are allergenic only upon their ingestion as a food source. Allergenic proteins of pollen are exposed to human immune system after their rapid release from pollen upon coming in contact with moist surface of nasal mucosa. The advent of molecular cloning and ability to genetically transform plants now offer unprecedented opportunities to produce hypoallergenic plants by targeted switching off allergen production. Gene silencing strategies that operate at post-transcriptional level are highly suitable for blocking allergen production. We have demonstrated the concept of allergen gene silencing through antisense approach by producing ryegrass plants that do not produce major allergen in its pollen. Our results show the potential of antisense approach in reducing the allergenic potential of plants. Such a strategy can have a general applicability for production of transgenic plants depleted of both inhaled and ingested allergens. In addition, such an approach could also help in elucidating the in vivo function of allergen(s) in plants and contribution of an allergen to overall allergenic potential of an allergen source.


Antisense downregulation; Antisense RNA; Hypoallergenic plants; Pollen allergen; Genetic engineering; Type 1 allergy; Grass; Transgenic plants; Gene silencing; allergenicity


Funding source
  • Australian Research Council
  • University of Melbourne
Funding country
  • Australia
Funding type
  • government
  • NGO: independent

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Cite this study


Bhalla, PL, MB Singh. "Knocking out expression of plant allergen genes." Methods 32.3 (2004): 340-5. Web. 24 Apr. 2024.


Bhalla, PL., & Singh, MB. (2004). Knocking out expression of plant allergen genes. Methods, 32(3), 340-5. doi:10.1016/j.ymeth.2003.08.011

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