Maghuly, Fatemeh; da Câmara Machado, Artur; Leopold, Stephan; Khan, Mahmood Ali; Katinger, Hermann; Laimer, Margit
Journal of Biotechnology. 2007 January. 127(2):310-321
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PMID: 16889860 DOI: 10.1016/j.jbiotec.2006.06.016 ISSN: 0168-1656
Transgenic trees currently are being produced by Agrobacterium-mediated transformation and biolistics. Since trees are particularly suited for long-term evaluations of the impact of the technology, Prunus subhirtella autumnorosa (PAR) was chosen as model fruit tree species and transformed with a reporter gene (uidA) under the control of the 35S promoter. Using Southern and GUS fluorometric techniques, we compared transgene copy numbers and observed stability of transgene expression levels in 34 different transgenic plants, grown under in vitro, greenhouse and screenhouse conditions, over a period of 9 years. An influence of grafting on gene expression was not observed. No silenced transgenic plant was detected. Overall, these results suggest that transgene expression in perennial species, such as fruit trees, remains stable in time and space, over extended periods and in different organs, confirming the value of PAR as model species to study season-dependent regulation in mature stonefruit tissues. While the Agrobacterium-derived Prunus transformants contained one to two copies of the transgenes, 91% of the transgenic events also contained various lengths of the bacterial plasmid backbone, indicating that the Agrobacterium-mediated transformation is not as precise as previously perceived. The implications for public acceptance and future applications are discussed.
Maghuly, Fatemeh, Artur da Câmara Machado, Stephan Leopold, Mahmood Ali Khan, Hermann Katinger, Margit Laimer. "Long-term stability of marker gene expression in Prunus subhirtella: A model fruit tree species." Journal of Biotechnology 127.2 (2007): 310-321. Web. 19 Nov. 2017.
Maghuly, Fatemeh., da Câmara Machado, Artur., Leopold, Stephan., Khan, Mahmood Ali., Katinger, Hermann., & Laimer, Margit. (2007). Long-term stability of marker gene expression in Prunus subhirtella: A model fruit tree species. Journal of Biotechnology, 127(2), 310-321. doi:10.1016/j.jbiotec.2006.06.016
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