There are currently 402 studies in the GENERA database.

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General Information

Document type
  • Peer-reviewed journal article
GE organism
  • rice
GE trait
  • insect resistance
Country
  • Denmark, UK, China

Results

Safety for consumption
  • mixed

Immunotoxicological studies of genetically modified rice expressing PHA-E lectin or Bt toxin in Wistar rats

Kroghsbo, S; Madsen, C; Poulsen, M; Schrøder, M; Kvist, PH; Taylor, M; Gatehouse, A; Shu, Q; Knudsen, I
Toxicology. 2008 March. 245(2-Jan):24–34

Link to full text (journal may charge for access)

PMID: 18215453 DOI: 10.1016/j.tox.2007.12.005 ISSN: 0300-483X

Abstract

As part of the SAFOTEST project the immunmodulating effect of Cry1Ab protein from Bacillus thuringiensis (Bt) and PHA-E lectin from kidney bean (Phaseolus vulgaris erythroagglutinin) was examined in 28- and 90-day feeding studies in Wistar rats. PHA-E lectin was chosen as positive control. Rats were fed control rice, transgenic rice expressing Cry1Ab protein or PHA-E lectin, or transgenic rice spiked with the purified recombinant protein. Total immunoglobulin levels, mitogen-induced cell proliferation, T-dependent antibody response to sheep red blood cells and the antigen-specific antibody response in serum were examined at the end of the studies. A dose-dependent increase in mesenteric lymph node weight and total immunoglobulin A was seen when feeding PHA-E transgenic rice alone or spiked with 0.1% purified PHA-E lectin for 90 days indicating a local effect of PHA-E in the intestine. No adverse effects of Cry1Ab protein were found. An anti-PHA-E and anti-Cry1Ab antibody response was induced both after inhalation (control groups) and after inhalation/ingestion (groups fed recombinant protein alone or together with transgenic rice). In conclusion, only PHA-E lectin was found to have an immunomodulating effect when feeding rats for 90 days with approximately 70 mg PHA-E/kg bodyweight per day. As both PHA-E lectin and Cry1Ab protein were capable of inducing an antigen-specific antibody response it is important to make careful considerations when designing future animal studies to avoid intake of proteins from the other groups by inhalation as well as to examine the sensitization and elicitation potential of 'foreign' proteins before introduction to the world market.

Keywords

Phaseolus vulgaris erythroagglutinin; Bacillus thuringiensis Cry1Ab protein; Immunotoxicology; Genetically modified rice; Wistar rats; SAFOTEST; PHA-E lectin

Funding

Funding source
  • European Commission
Funding country
  • EU
Funding type
  • government

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Cite this study

MLA

Kroghsbo, S, C Madsen, M Poulsen, M Schrøder, PH Kvist, M Taylor, A Gatehouse, Q Shu, I Knudsen. "Immunotoxicological studies of genetically modified rice expressing PHA-E lectin or Bt toxin in Wistar rats." Toxicology 245.2-Jan (2008): 24–34. Web. 25 Sep. 2017.

APA

Kroghsbo, S., Madsen, C., Poulsen, M., Schrøder, M., Kvist, PH., Taylor, M., Gatehouse, A., Shu, Q., & Knudsen, I. (2008). Immunotoxicological studies of genetically modified rice expressing PHA-E lectin or Bt toxin in Wistar rats. Toxicology, 245(2-Jan), 24–34. doi:10.1016/j.tox.2007.12.005

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