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General Information

Document type
  • Peer-reviewed journal article
GE organism
  • soybean
GE trait
  • herbicide tolerance
Country
  • USA

Results

Equivalence
  • no effect
Safety for consumption
  • no effect

Determining whether transgenic and endogenous plant DNA and transgenic protein are detectable in muscle from swine fed Roundup Ready soybean meal Open Access

Jennings, JC; Kolwyck, DC; Kays, SB; Whetsell, AJ; Surber, JB; Cromwell, GL; Lirette, RP; Glenn, KC
Journal of Animal Science. 2003 June. 81(6):1447-1455

Link to full text (open access, freely available)

PMID: 12817492 ISSN: 0021-8812

Abstract

Questions regarding the digestive fate of DNA and protein from transgenic feed have been raised in regard to human consumption and commercial trade of animal products (e.g., meat, milk, and eggs) from farm animals fed transgenic crops. Using highly sensitive, well-characterized analytical methods, pork loin samples were analyzed for the presence of fragments of transgenic and endogenous plant DNA and transgenic protein from animals fed meal prepared from conventional or glyphosate-tolerant Roundup Ready (RR) soybeans. Pigs were fed diets containing 24, 19, and 14% RR or conventional soybean meal during grower, early-finisher, and late-finisher phases of growth, respectively, and longissimus muscle samples were collected (12 per treatment) after slaughter. Total DNA was extracted from the samples and analyzed by PCR, followed by Southern blot hybridization for the presence of a 272-bp fragment of the cp4 epsps coding region (encoding the synthetic enzyme 5-enolpyruvylshikimate-3-phosphate synthase derived from Agrobacterium sp. strain CP4) and a 198-bp fragment of the endogenous soybean gene le1 (encoding soy lectin). Using 1 microgram of input DNA per reaction, none of the extracted samples was positive for cp4 epsps or le1 at the limit of detection (LOD) for these PCR/Southern blot assays. The LOD for these assays was shown to be approximately one diploid genome equivalent of RR soybean DNA, even in the presence of 10 micrograms of pork genomic DNA. A 185-bp fragment of the porcine preprolactin (prl) gene, used as a positive control, was amplified from all samples showing that the DNA preparations were amenable to PCR amplification. Using a competitive immunoassay with an LOD of approximately 94 ng of CP4 EPSPS protein/g of pork muscle, neither the CP4 EPSPS protein nor the immunoreactive peptide fragments were detected in loin muscle homogenates from pigs fed RR soybean meal. Taken together, these results show that neither small fragments of transgenic DNA nor immunoreactive fragments of transgenic protein are detectable in loin muscle samples from pigs fed a diet containing RR soybean meal.

Keywords

Biotechnology; Enzyme-Linked Immunosorbent Assay; Pigs; Polymerase Chain Reaction; Soybeans; event 40-30-2

Funding

Funding source
  • Monsanto
Funding country
  • United States
Funding type
  • industry: same

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Cite this study

MLA

Jennings, JC, DC Kolwyck, SB Kays, AJ Whetsell, JB Surber, GL Cromwell, RP Lirette, KC Glenn. "Determining whether transgenic and endogenous plant DNA and transgenic protein are detectable in muscle from swine fed Roundup Ready soybean meal." Journal of Animal Science 81.6 (2003): 1447-1455. Web. 25 Jul. 2017.

APA

Jennings, JC., Kolwyck, DC., Kays, SB., Whetsell, AJ., Surber, JB., Cromwell, GL., Lirette, RP., & Glenn, KC. (2003). Determining whether transgenic and endogenous plant DNA and transgenic protein are detectable in muscle from swine fed Roundup Ready soybean meal. Journal of Animal Science, 81(6), 1447-1455.

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