There are currently 402 studies in the GENERA database.

Advanced Search

General Information

Document type
  • Journal article
GE organism
  • maize
GE trait
  • insect resistance
Country
  • Spain

Results

Equivalence
  • mixed

Analysis of chiral amino acids in conventional and transgenic maize

Herrero, M; Ibáñez, E; Martín-Alvarez, PJ; Cifuentes, A
Analytical Chemistry . 2007 May . 79(13):5071-7

Link to full text (journal may charge for access)

PMID: 17523597 DOI: 10.1021/ac070454f

Abstract

In this work, a new chiral micellar electrokinetic chromatography with laser-induced fluorescence detection (chiral-MEKC-LIF) method is proposed to identify and quantify D- and L-amino acids in three lines of transgenic maize and their corresponding nontransgenic parental lines grown under identical conditions. The optimized procedure includes amino acids extraction, derivatization with FITC and chiral-MEKC-LIF separation in a background electrolyte composed of 100 mM sodium tetraborate, 80 mM SDS, and 20 mM beta-CD at pH 10.0. The D- and L-forms of Arg, Ser, Ala, Glu, and Asp, corresponding to the majority amino acids usually found in maize, are separated in less than 25 min with efficiencies up to 890,000 plates/m and high sensitivity (i.e., LODs as low as 160 nM were obtained for D-Arg for a signal-to-noise ratio of three), allowing the detection of 1% D-Arg in the presence of 99% of its opposite enantiomer. Using this method, different D-amino acids are detected in all investigated maize samples providing the reproducible quantification of the D-enantiomeric excess (% d-aa) for each amino acid calculated as % D-aa = 100D-aa/(D-aa + L-aa). Thus, significant differences were observed among the % d-aa values for the different conventional varieties (Aristis, Tietar, and PR33P66 maize) as could be expected from their natural variability. More interestingly, comparing each conventional maize with its corresponding transgenic line, very similar % D-aa values were obtained for one of the studied maize couples (Tietar vs Tietar-Bt) what could be presented as a new proof of their substantial equivalence. However, significant differences in the % d-aa values were observed for the other lines of maize studied. It is concluded that enantioselective procedures can open new perspectives in the study of transgenic organisms in order to corroborate (or not) the equivalence with their conventional counterparts.

Keywords

amino acids; maize corn; chirality

Funding

Funding source
  • Ministry of Education and Science
Funding country
  • Spain
Funding type
  • government

Links to outside analysis of this resource

Please contact us if you know of an independent summary or analysis of this resource.

Cite this study

MLA

Herrero, M, E Ibáñez, PJ Martín-Alvarez, A Cifuentes. "Analysis of chiral amino acids in conventional and transgenic maize ." Analytical Chemistry 79.13 (2007): 5071-7 . Web. 27 Jul. 2017.

APA

Herrero, M., Ibáñez, E., Martín-Alvarez, PJ., & Cifuentes, A. (2007). Analysis of chiral amino acids in conventional and transgenic maize . Analytical Chemistry , 79(13), 5071-7 . doi:10.1021/ac070454f

Please verify citations before use, citations are automatically generated based on information stored within the GENERA database and therefore may or may not be correct.