There are currently 402 studies in the GENERA database.

Advanced Search

General Information

Document type
  • Peer-reviewed journal article
GE organism
  • maize
GE trait
  • insect resistance
Country
  • Germany, Switzerland

Results

Safety for consumption
  • no effect

Fate of recombinant DNA and Cry1Ab protein after ingestion and dispersal of genetically modified maize in comparison to rapeseed by fallow deer (Dama dama)

Guertler, P; Lutz, B; Kuehn, R; Meyer, HHD; Einspanier, R; Killermann, B; Albrecht, C
European Journal of Wildlife Research. 2008 February. 54(1):36-43

Link to full text (journal may charge for access)

DOI: 10.1007/s10344-007-0104-4 ISSN: 1612-4642

Abstract

The fate of recombinant DNA in fallow deer (Dama dama) was investigated by feeding a diet of isogenic or genetically modified (GM) maize expressing Cry1Ab protein against the European corn borer (Ostrinia nubilalis). To study the degradability of ingested DNA, polymerase chain reaction (PCR) assays were introduced to detect fragments of the endogenous, highly abundant chloroplast-specific rubisco gene, the maize-specific zein gene and the recombinant cry1Ab gene. PCR analysis revealed that small chloroplast- and maize-specific DNA fragments were detectable in contents of rumen, abomasums, jejunum, caecum and colon and occasionally in visceral tissues. In contrast, no fragments of the recombinant cry1Ab gene were detectable in gastrointestinal (GI) contents. The Cry1Ab protein was analysed using an enzyme-linked immunosorbent assay (ELISA) and immunoblotting technique. Neither ELISA nor immunoblotting yielded positive signals of immunoactive Cry1Ab protein in GI contents and tissues of fallow deer fed with GM maize. In conclusion, after uptake of GM maize, neither cry1Ab-specific gene fragments nor Cry1Ab protein were detected in the GI tract of fallow deer, indicating complete digestion of the GM maize. Additional investigations on the germination capacity of conventional rapeseed and maize seed after ingestion by fallow deer and faecal excretion (endozoochory) were performed to draw conclusions regarding a potential spreading of germinable GM crop seed by deer. Germination tests revealed that germinable rapeseed kernels were detectable in faeces; in contrast, no intact maize seeds were found in faeces.

Keywords

Genetically modified plants; Bt-maize; MON810; Bt176; Wildlife

Funding

Funding source
  • Federal Agency for Nature Conservation
Funding country
  • Germany
Funding type
  • government

Links to outside analysis of this resource

Please contact us if you know of an independent summary or analysis of this resource.

Cite this study

MLA

Guertler, P, B Lutz, R Kuehn, HHD Meyer, R Einspanier, B Killermann, C Albrecht. "Fate of recombinant DNA and Cry1Ab protein after ingestion and dispersal of genetically modified maize in comparison to rapeseed by fallow deer (Dama dama) ." European Journal of Wildlife Research 54.1 (2008): 36-43. Web. 20 Nov. 2017.

APA

Guertler, P., Lutz, B., Kuehn, R., Meyer, HHD., Einspanier, R., Killermann, B., & Albrecht, C. (2008). Fate of recombinant DNA and Cry1Ab protein after ingestion and dispersal of genetically modified maize in comparison to rapeseed by fallow deer (Dama dama) . European Journal of Wildlife Research, 54(1), 36-43. doi:10.1007/s10344-007-0104-4

Please verify citations before use, citations are automatically generated based on information stored within the GENERA database and therefore may or may not be correct.