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General Information

Document type
  • Peer-reviewed journal article
GE organism
  • potato
GE trait
  • insect resistance
Country
  • Egypt

Results

Safety for consumption
  • mixed

Fine structural changes in the ileum of mice fed on delta-endotoxin-treated potatoes and transgenic potatoes

Fares, NH; El-Sayed, AK
Natural Toxins. 1998 November/December. 6(6):219-33

Link to full text (journal may charge for access)

DOI: 10.1002/(SICI)1522-7189(199811/12)6:6<219::AID-NT30>3.0.CO;2-K ISSN: 1522-7189

Abstract

The present work has been designed to study the effect of feeding on transgenic potatoes, which carry the CryI gene of Bacillus thuringiensis var. kurstaki strain HD1, on the light and electron microscopic structure of the mice ileum, in comparison with feeding on potatoes treated with the ‘δ-endotoxin’ isolated from the same bacterial strain. The microscopic architecture of the enterocytes of the ileum of both groups of mice revealed certain common features such as the appearance of mitochondria with signs of degeneration and disrupted short microvilli at the luminal surface. However, in the group of mice fed on the ‘δ-endotoxin’, several villi appeared with an abnormally large number of enterocytes (151.8 in control group versus 197 and 155.8 in endotoxin and transgenic-treated groups, respectively). Fifty percent of these cells were hypertrophied and multinucleated. The mean area of enterocyte was significantly increased (105.3 µm2 in control group versus 165.4 µm2 and 116.5 µm2 in endotoxin and transgenic-treated groups, respectively). Several forms of secondary lysosomes or auotophagic vacuoles were recognized in these cells. These changes were confirmed with the scanning electron microscope which revealed a remarkable increase in the topographic contour of enterocytes (23 µm in control group versus 44 µm and 28 µm in endotoxin and transgenic-treated groups, respectively) at the divulged surface of the villi. The basal lamina along the base of the enterocytes was damaged at several foci. Several disrupted microvilli appeared in association with variable-shaped cytoplasmic fragments. Some of these fragments contained endoplasmic reticulum, as well as ring-shaped annulate lamellae. In addition, the Paneth cells were highly activated and contained a large number of secretory granules. These changes may suggest that δ-endotoxin-treated potatoes resulted in the development of hyperplastic cells in the mice ileum. Although mild changes are reported in the structural configuration of the ileum of mice fed on transgenic potatoes, nevertheless, thorough tests of these new types of genetically engineered crops must be made to avoid the risks before marketing.

Keywords

scanning; ultrastructure; ileum; Bacillus thuringiensis var. kurstaki; transgenic potatoes; δ-endotoxin

Funding

Funding source
  • Not reported
Funding country
  • Not reported
Funding type
  • Not reported

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Cite this study

MLA

Fares, NH, AK El-Sayed. "Fine structural changes in the ileum of mice fed on delta-endotoxin-treated potatoes and transgenic potatoes." Natural Toxins 6.6 (1998): 219-33. Web. 19 Sep. 2017.

APA

Fares, NH., El-Sayed, AK. (1998). Fine structural changes in the ileum of mice fed on delta-endotoxin-treated potatoes and transgenic potatoes. Natural Toxins, 6(6), 219-33. doi:10.1002/(SICI)1522-7189(199811/12)6:6<219::AID-NT30>3.0.CO;2-K

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