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General Information

Document type
  • Peer-reviewed journal article
GE organism
  • tobacco
GE trait
  • disease resistance
Country
  • UK
  • Korea

Results

Safety for environment
  • no effect

Stability of recombinant plant viruses containing genes of unrelated plant viruses

Chung, BN; Canto, T; Palukaitis, P
Journal of General Virology. 2007 April. 88(4):1347-1355

Link to full text (journal may charge for access)

PMID: 17374781 DOI: 10.1099/vir.0.82477-0 ISSN: 0022-1317

Abstract

The stability of hybrid plant viruses that might arise by recombination in transgenic plants was examined using hybrid viruses derived from the viral expression vectors potato virus X (PVX) and tobacco rattle virus (TRV). The potato virus Y (PVY) NIb and HCPro open reading frames (ORFs) were introduced into PVX to generate PVX-NIb and PVX-HCPro, while the PVY NIb ORF was introduced into a vector derived from TRV RNA2 to generate TRV-NIb. All three viruses were unstable and most of the progeny viruses had lost the inserted sequences between 2 and 4 weeks post-inoculation. There was some variation in the rate of loss of part or all of the inserted sequence and the number of plants containing the deleted viruses, depending on the sequence, the host (Nicotiana tabacum vs Nicotiana benthamiana) or the vector, although none of these factors was associated consistently with the preferential loss of the inserted sequences. PVX-NIb was unable to accumulate in NIb-transgenic tobacco resistant to infection by PVY and also showed loss of the NIb insert from PVX-NIb in some NIb-transgenic tobacco plants susceptible to infection by PVY. These data indicate that such hybrid viruses, formed in resistant transgenic plants from a transgene and an unrelated virus, would be at a selective disadvantage, first by being targeted by the resistance mechanism and second by not being competitive with the parental virus.

Keywords

virus; selective disadvantage; tobacco; potato virus; viral recombination

Funding

Funding source
  • European Commission
  • Korean Rural Development Administration
  • Korea Science and Engineering Foundation
Funding country
  • EU
  • Korea
Funding type
  • government

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Cite this study

MLA

Chung, BN, T Canto, P Palukaitis. "Stability of recombinant plant viruses containing genes of unrelated plant viruses." Journal of General Virology 88.4 (2007): 1347-1355. Web. 25 Sep. 2017.

APA

Chung, BN., Canto, T., & Palukaitis, P. (2007). Stability of recombinant plant viruses containing genes of unrelated plant viruses. Journal of General Virology, 88(4), 1347-1355. doi:10.1099/vir.0.82477-0

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