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General Information

Document type
  • Peer-reviewed journal article
GE organism
  • maize
GE trait
  • insect resistance
Country
  • Germany

Results

Safety for consumption
  • no effect

Impact of Bacillus thuringiensis toxin Cry1Ab on rumen epithelial cells (REC) - a new in vitro model for safety assessment of recombinant food compounds

Bondzio, A; Stumpff, F; Schön, J; Martens, H; Einspanier, R
Food and Chemical Toxicology. 2008 June. 46(6):1976–1984

Link to full text (journal may charge for access)

PMID: 18325653 DOI: 10.1016/j.fct.2008.01.038 ISSN: 0278-6915

Abstract

The growing use of genetically modified crops necessitates viable screening methods for safety evaluation of recombinant feed, particularly for ruminants. A new sheep rumen epithelial cell culture is introduced as an in vitro cell system for safety evaluation especially focussing on feed and food compounds. We used lactate dehydrogenase (LDH) release, WST-1 conversion, ATP content and caspase 3/7 activity to evaluate cytotoxicity of Cry1Ab, one of the newly expressed Bt-proteins in transgene maize. The results were compared to the effects of valinomycin, a potassium ionophore known to induce cytotoxic effects on a wide range of cells. Whereas no toxicity of Cry1Ab was observed in short as well as in long term experiments, even at non-physiological high concentrations, exposure to valinomycin induced apoptosis and a significant response of all viability parameters after a number of hours. The ATP content and the WST-1 conversion reflecting the energy metabolism of the cells appear to be more sensitive indicators of valinomycin toxicity than the LDH release, a parameter which reflects the membrane integrity. This study presents an in vitro model system, that may be useful as a supplementary tool in toxicity screening before testing substances on animals in vivo.

Keywords

Cry1Ab protein; Rumen epithelial cells; Valinomycin; Cytotoxicity assays

Funding

Funding source
  • Federal Ministry of Education and Research
  • Margarete-Marcus Charity
Funding country
  • Germany
Funding type
  • government
  • NGO: independent

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Cite this study

MLA

Bondzio, A, F Stumpff, J Schön, H Martens, R Einspanier. "Impact of Bacillus thuringiensis toxin Cry1Ab on rumen epithelial cells (REC) - a new in vitro model for safety assessment of recombinant food compounds." Food and Chemical Toxicology 46.6 (2008): 1976–1984. Web. 25 Sep. 2017.

APA

Bondzio, A., Stumpff, F., Schön, J., Martens, H., & Einspanier, R. (2008). Impact of Bacillus thuringiensis toxin Cry1Ab on rumen epithelial cells (REC) - a new in vitro model for safety assessment of recombinant food compounds. Food and Chemical Toxicology, 46(6), 1976–1984. doi:10.1016/j.fct.2008.01.038

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